[1]郭永月,陶明煊,赵云霞,等.白玉菇多糖提取方法的比较和优化[J].南京师大学报(自然科学版),2013,36(03):87-92.
　Guo Yongyue,Tao Mingxuan,Zhao Yunxia,et al.Comparison and Optimization Among Extraction Technologies of Polysaccharide From Pleurotus Nebrodensis[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(03):87-92.
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白玉菇多糖提取方法的比较和优化()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年03期
页码:
87-92
栏目:
生命科学
出版日期:
2013-09-30

文章信息/Info

Title:
Comparison and Optimization Among Extraction Technologies of Polysaccharide From Pleurotus Nebrodensis
作者:
郭永月; 陶明煊; 赵云霞; 丁伶俐
南京师范大学金陵女子学院,江苏 南京 210097
Author(s):
Guo Yongyue; Tao Mingxuan; Zhao Yunxia; Ding Lingli
Ginling College,Nanjing Normal University,Nanjing 210097,China
关键词:
白玉菇; 多糖提取; 超声波; 微波; 碱提; 响应面
Keywords:
Pleurotus nebrodensis; polysaccharides extraction; ultrasound; microwave; ulkali mention; response surface methodology
分类号:
R284.2
摘要:
优选白玉菇多糖的提取工艺.采用热水浸提法、超声波辅助提取法、微波辅助提取法和碱提法4种方法提取白玉菇多糖,并采用响应面法优化超声波辅助提取法.热水浸提法、超声波辅助提取法、微波辅助提取法和碱提法提取多糖得率分别为2.90%、5.48%、4.86%、4.71%.超声波辅助提取法提取白玉菇多糖的得率与其他3种方法比较有显著差异.响应面法优化超声波辅助提取法的最优条件为:液料比23:1,超声时间28 min(300 W),在92 ℃下热水浸提2 h,重复3次,测定白玉菇多糖的得率为6.03%.超声波辅助提取法可以显著提高白玉菇多糖得率.
Abstract:
The purpose of the experiment is to choose the best extraction technology of polysaccharide from Pleurotus nebrodensis.The Pleurotus nebrodensis was used as the raw material for extracting polysaccharide by means of hot water extraction,ultrasonic-assisted extraction,microwave-assisted extraction,ulkali mention.A central composite design followed by response surface analysis was carried out to optimize the ultrasonic-assisted extraction parameters.The yields of the four extraction technologies were 2.9%,5.48%,4.86% and 4.71%.The results of determination of polysaccharide content showed that ultrasonic-assisted extraction had significant differences in the four extraction technologies.The optimal ultrasonic-assisted extraction conditions were 28 min at ultrasonic power 300 W with a water-material ratio of 23:1 and three times repeated extractions at 92 ℃ for 2 h each time,which yielded an extraction yield of 6.03%.The ultrasonic-assisted extraction could significantly improve the yield of polysaccharide from Pleurotus nebrodensis.

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备注/Memo

备注/Memo:
收稿日期:2012-10-19.
基金项目:江苏省高校自然科学基础研究项目(10KJD550003)、江苏省普通高校研究生科研创新计划项目(CXLX11_0895).
通讯联系人:陶明煊,副教授,研究方向:生物活性物质与保健功能因子.E-mail:45017@njnu.edu.cn
更新日期/Last Update: 2013-09-30