[1]袁秀雪,陈恩懿,肖梦思,等.光谱法比较光疗药物竹红菌甲素及铂竹红菌甲素复合物与牛血红蛋白的相互作用(英文)[J].南京师范大学学报(自然科学版),2015,38(03):14.
 YuanXiuxue,ChenEnyi,XiaoMengsi,et al.ComparingtheInteractionofPlatinum-HypocrellinAComplexandHypocrellinAwithBovineHemoglobin[J].Journal of Nanjing Normal University(Natural Science Edition),2015,38(03):14.
点击复制

光谱法比较光疗药物竹红菌甲素及铂竹红菌甲素复合物与牛血红蛋白的相互作用(英文)()
分享到:

《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第38卷
期数:
2015年03期
页码:
14
栏目:
化学
出版日期:
2015-09-30

文章信息/Info

Title:
ComparingtheInteractionofPlatinum-HypocrellinAComplexandHypocrellinAwithBovineHemoglobin
作者:
袁秀雪陈恩懿肖梦思赵传峰周家宏
南京师范大学化学与材料科学学院,分析测试中心,江苏省生物功能材料重点实验室,江苏省生物功能材料协同创新中心,应用光化学重点实验室,江苏南京210023
Author(s):
YuanXiuxueChenEnyiXiaoMengsiZhaoChuanfengZhouJiahong
SchoolofChemistryandMaterialsScience,AnalysisandTestingCentre,JiangsuKeyLaboratoryofBiofunctionalMaterials,JiangsuCollaborativeInnovationCentreofBiomedicalFunctionalMaterials,KeyLaboratoryofAppliedPhotochemistry,NanjingNormalUniversity,Nanjing210023,China
关键词:
竹红菌甲素铂竹红菌甲素复合物牛血红蛋白相互作用
Keywords:
hypocrellinAplatinum-hypocrellinAbovinehemoglobininteraction
分类号:
O657.3
文献标志码:
A
摘要:
通过紫外-可见吸收光谱、瞬态荧光光谱以及圆二色谱来比较光疗药物竹红菌甲素及铂竹红菌甲素复合物与牛血红蛋白的相互作用.结果表明,虽然竹红菌甲素及铂竹红菌甲素复合物与牛血红蛋白的反应机理都是基于电子转移的静态猝灭,但是铂竹红菌甲素复合物与牛血红蛋白的反应不仅要比竹红菌甲素强,而且反应后牛血红蛋白的微环境和构像的改变也要大于竹红菌甲素.
Abstract:
TheinteractionmechanismsofHypocrellinA(HA)anditsplatinumcomplex(Pt-HA)withbovinehemoglobin(BHb)werecomparedbyultraviolet-visible(UV-Vis)absorption,time-resolvedfluorescencespectra,circulardichroism(CD)andinducedcirculardichroism(ICD)spectra,respectively.TheUV-VisresultsrevealedthatbothHAandPt-HAcouldinteractwithBHbandthelatterinteractionwasstrongerthanHA.Inaddition,quenchingmechanismofHAandPt-HAwerebothstaticprocess,whichwereascribedtotheelectrontransfer.Moreover,CDandICDspectrumrevealedthatthemicroenvironmentandconformationofBHbwerechangedafterconjugationwithHAandPt-HA,asthelatteralteredmoreacute.Finally,thecomparisonresultsdemonstratedthattheinteractionofPt-HAandBHbwasstrongerandmorestablethanHA.

参考文献/References:

[1]ShiJJ,WangL,GaoJ,etal.Afullerene-basedmulti-functionalnanoplatformforcancertheranosticapplications[J].Biomate,2014,35(22):5771-5784.
[2]GormanA,KilloranJ,O’SheaC,etal.Invitrodemonstrationoftheheavy-atomeffectforphotodynamictherapy[J].JAmChemSoc,2004,126(34):10619-10631.
[3]YangC,MaF,TangJ,etal.Synthesisofvanadyl-hypocrellinacomplexanditsphotodynamicpropertiesresearch[J].BioorgMedChemLett,2012,22(15):5003-5007.
[4]ZhouL,LiuJH,MaF,etal.Mitochondria-targetingphotosensitizer-encapsulatedamorphousnanocageasabimodalreagentfordrugdeliveryandbiodiagnoseinvitro[J].BiomedMicrodevices,2010,12(4):655-663
[5]MaF,GeXF,HuangHY,etal.InteractionsofCT-DNAwithhypocrellinaanditsAl3+-HypocrellinAcomplex[J].SpectrochimActaPartA,2013,109:158-163.
[6]XiaoMS,HanL,ZhouL,etal.Comparisonandinvestigationofbovinehemoglobinbindingtodihydroartemisininand9-hydroxy-dihydroartemisinin:spectroscopiccharacterization[J].SpectrochimActaPartA,2014,125:120-125.
[7]WuY,CuiW,ZhouS,etal.Thebindingbehaviorofitraconazolewithhemoglobin:studiesfrommulti-spectroscopictechniques[J].SpectrochimActaPartA,2014,131:407-412.
[8]DangXJ,NieMY,TongJ,etal.Inclusionof10-methylphenothiazineanditselectrochemicallygeneratedcationradicalbyβ-cyclodextrininwater+methanolsolventmixtures[J].JElectroanalChem,1997,437(1):53-59.
[9]SunY,JiZ,LiangX,,etal,Studiesonthebindingofrhaponticinwithhumanserumalbuminbymolecularspectroscopy,modelingandequilibriumdialysis[J].SpectrochimActaPartA,2012,87:171-178.
[10]BakkialakshmiS,ChandrakalaD.Aspectroscopicinvestigationsofanticancerdrugsbindingtobovineserumalbumin[J].SpectrochimActaPartA,2012,88:2-9.
[11]PradhanA,PalP,DurocherG,etal.Steadystateandtime-resolvedfluorescencepropertiesofmetastaticandnon-metastaticmalignantcellsfromdifferentspecies[J].JPhotochemPhotobiol,1995,31(3):101-112.
[12]SentchoukVV,BodaryukEV.Fluorescentanalysisofinteractionofflavonolswithhemoglobinandbovineserumalbumin[J].JApplSpectrosc,2007,74(5):731-737.
[13]WangYQ,ZhangHM,ZhouQH.Studiesontheinteractionofcaffeinewithbovinehemoglobin[J].EurJMedChem,2009,44(5):2100-2105.
[14]TangJ,YangC,ZhouL,etal.Studiesonthebindingbehaviorofprodigiosinwithbovinehemoglobinbymulti-spectroscopictechniques[J].SpectrochimActaPartA,2012,96(2012):461-467.
[15]AshokaS,SeetharamappaJ,KandagalPB,etal.Investigationoftheinteractionbetweentrazodonehydrochlorideandbovineserumalbumin[J].JLumin,2006,121(1):179-186.
[16]WangYQ,ZhangHM,ZhangGC,etal.Studiesoftheinteractionbetweenparaquatandbovinehemoglobin[J].IntJBiolMacromol,2007,41(3):243-250.
[17]KandagalPB,ShaikhSMT,ManjunathaDH,etal.Spectroscopicstudiesonthebindingofbioactivephenothiazinecompoundstohumanserumalbumin[J].JPhotochemPhotobiol,2007,189(1):121-127.
[18]IbrahimMS.VoltammetricstudiesoftheinteractionofnogalamycinantitumordrugwithDNA[J].AnalChimAct,2001,443(1):63-72.
[19]XiaoJ,ShiJ,CaoH,etal.Analysisofbindinginteractionbetweenpuerarinandbovineserumalbuminbymulti-spectroscopicmethod[J].JPharmBiomedAnal,2007,45(4):609-615
[20]Gonzalez-JimenezJ,CortijoM.Urea-induceddenaturationofhumanserumalbuminlabeledwithacrylodan[J].JProteinChem,2002,21(2):75-79.
[21]ShahabadiN,KashanianS,DarabiF.InvitrostudyofDNAinteractionwithawater-solubledinitrogenschiffbase[J].DNACellBiol,2009,28(11):589-596.
[22]ShahabadiN,FatahiA.MultispectroscopicDNA-bindingstudiesofatris-chelatenickel(II)complexcontaining4,7-diphenyl1,10-phenanthrolineligands[J].JMolStruct,2010,970(1):90-95.
[23]GuoXJ,JingK,GuoC,etal.Theinvestigationoftheinteractionbetweenoxybutyninhydrochlorideandbovineserumalbuminbyspectroscopicmethods[J].JLumin,2010,130(12):2281-2287.
[24]ZhouJH,WuXH,GuXT,etal.SpectroscopicstudiesontheinteractionofhypocrellinAandhemoglobin[J].SpectrochimActaPartA,2009,72(1):151-155.
[25]MahatoM,PalP,KamilyaT,etal.Hemoglobin-silverinteractionandbioconjugateformation:aspectroscopicstudy[J].JPhysChemB,2010,114(20):7062-7070.
[26]YamashitaS,NishimotoE,SzaboAG,etal.Steady-stateandtime-resolvedfluorescencestudiesontheligand-inducedconformationalchangeinanactivelysozymederivative,Kyn62-Lysozyme[J].Biochem,1996,35(2):531-537.
[27]SzaboAG,KrajcarskiD,ZukerM.Conformationalheterogeneityinhemoglobinasdeterminedbypicosecondfluorescencedecaymeasurementsofthetryptopranresidues[J].ChemPhysLett,1984,108(2):145-149.
[28]ChaudhuriS,ChakrabortyS,SenguptaPK.Probingtheinteractionsofhemoglobinwithantioxidantflavonoidsviafluorescencespectroscopyandmolecularmodelingstudies[J].BiophysChem,2011,154(1):26-34.
[29]BairdS,KellySM,PriceNC,etal.HemocyaninconformationalchangesassociatedwithSDS-inducedphenoloxidaseactivation[J].JNairnBiochimBiophysActa,ProteinsProteomics,2007,174(11):1380-1394.
[30]BiSY,YanLL,WangBB,etal.Spectroscopicandvoltammetriccharacterizationsoftheinteractionoftwolocalanestheticswithbovineserumalbumin[J].JLumin,2011,131(5):866-873.
[31]YuP,WenXM,TohYR,etal,Efficientelectrontransferincarbonnanodot-grapheneoxidenanocomposites[J].JMaterChemC,2014,2(16):2894-2901.

[33]LiR,NagaiY,NagaiM.Changesoftyrosineandtryptophanresiduesinhumanhemoglobinbyoxygenbinding:near-andfar-UVcirculardichroismofisolatedchainsandrecombinedhemoglobin[J].JInorgBiochem,2000,82(1):93-101.
[33]SarehS,JamshidkhanC.Investigationontheinteractionbetweentamoxifenandhumanholo-transferrin:determinationofthebindingmechanismbyfluorescencequenching,resonancelightscatteringandcirculardichroismmethods[J].IntJBiolMacromol,2010,47(4):558-569.
[34]Bolanos-GarciaVM,RamosS,CastilloR,etal.Monolayersofapolipoproteinsattheair/waterinterface[J].JPhysChemB,2001,105(24):5757-5765.
[35]HuangBX,KimHY,DassC.Probingthree-dimensionalstructureofbovineserumalbuminbychemicalcross-linkingandmassspectrometry[J].JAmSocMassSpectrom,2004,15(8):1237-1247.
[36]AllenmarkS.Inducedcirculardichroismbychiralmolecularinteraction[J].Chirality,2003,15(5):409-422.
[37]MandalP,BardhanM,GangulyT,Spectroscopicinvestigationstorevealthenatureofinteractionsbetweenthehaemproteinmyoglobinandthedyerhodamine6G[J].Lumin,2012,27(4):285-291.

相似文献/References:

[1]王薇,周林,唐静,等.纳米TiO_2载体对竹红菌甲素光敏损伤DNA的增敏研究[J].南京师范大学学报(自然科学版),2011,34(04):60.
 Wang Wei,Zhou Lin,Tang Jing,et al.Photosensitization Studies of TiO2 Nanoparticles to Photocleavage Capacility of Hypocrellin A[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(03):60.
[2]赵传峰,周林,马菲,等.新型竹红菌甲素水溶性纳米二氧化硅载体的制备激光敏活性研究[J].南京师范大学学报(自然科学版),2010,33(04):177.
[3]宋开玺,周家宏,吴晓红,等.竹红菌甲素和溶菌酶之间的电子转移与荧光猝灭作用(英文)[J].南京师范大学学报(自然科学版),2008,31(02):59.
 Song Kaixi,Zhou Jiahong,Wu Xiaohong,et al.Affinity Interaction Between Lysozyme and Hyprocrellin A:Electron Transfer and Fluorescence Quench[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(03):59.
[4]顾晓天,吴晓红,周家宏,等.竹红菌甲素与细胞色素C相互作用的同步荧光光谱研究[J].南京师范大学学报(自然科学版),2005,28(04):70.
 Gu Xiaotian~,Wu Xiaohong~,Zhou Jiahong~,et al.Study on the Interaction Between Hypocrellin A and Cytochrome C Using Synchronous Fluorescence Spectra[J].Journal of Nanjing Normal University(Natural Science Edition),2005,28(03):70.
[5]周家宏,冯玉英,魏少华,等.竹红菌甲素和竹红菌乙素的光电转换性能[J].南京师范大学学报(自然科学版),2004,27(04):59.
 Zhou Jiahong,Feng Yuying,Wei Shaohua,et al.Study of Photoinduced Electron Transfer of Hypocrellin A and Hyprocrellin B[J].Journal of Nanjing Normal University(Natural Science Edition),2004,27(03):59.

备注/Memo

备注/Memo:
Receiveddata:2015-03-06.
Foundationitem:ProjectofDepartmentofScienceandTechnologyofJiangsuProvince(BK20131394、BZ201210).
Correspondingauthor:ZhaoChuanfeng,seniorengineer,majoredinappliedchemistry.E-mai:zhaochuanfeng@njnu.edu.cn
更新日期/Last Update: 2015-09-30