[1]王 倩*,潘 望,李 伟*,等.Blf-ADT对HeLa细胞的抑制作用及其机制的研究[J].南京师范大学学报(自然科学版),2016,39(01):67.
Wang Qian,Pan Wang,Li Wei,et al.Study on Anti-Tumor Activity and Mechanisms of Blf-ADT[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(01):67.
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Blf-ADT对HeLa细胞的抑制作用及其机制的研究()
《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]
- 卷:
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第39卷
- 期数:
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2016年01期
- 页码:
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67
- 栏目:
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生命科学
- 出版日期:
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2016-03-31
文章信息/Info
- Title:
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Study on Anti-Tumor Activity and Mechanisms of Blf-ADT
- 作者:
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王 倩*; 潘 望; 李 伟*; 肖志一; 潘黎黎; 绪广林
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南京师范大学生命科学学院,江苏省分子医学与生物技术重点实验室,江苏 南京 210023
- Author(s):
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Wang Qian; Pan Wang; Li Wei; Xiao Zhiyi; Pan Lili; Xu Guanglin
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School of Life Sciences,Nanjing Normal University,Jiangsu Province Key Laboratory for Molecularand Medical Biotechnology,Nanjing 210023,China
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- 关键词:
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HeLa; Blf-ADT; 细胞增殖; 细胞凋亡; 抗肿瘤作用
- Keywords:
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HeLa; Blf-ADT; cell proliferation; cell apoptosis; anti-tumor effect
- 分类号:
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Q4
- 文献标志码:
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A
- 摘要:
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研究Blf-ADT(布洛芬硫化氢供体物)的抗肿瘤作用,并对其机制作初步探讨.通过形态学观察、噻唑蓝(MTT)比色实验、集落形成实验测定HeLa细胞的增殖能力;细胞划痕实验测定HeLa细胞的迁移能力;Annexin V-FITC/PI双染流式细胞仪测定HeLa细胞的凋亡率;Western blotting检测细胞凋亡相关蛋白(Bcl-2、Bax、Erk、P38和Procaspase-3等)的表达.结果显示,Blf-ADT能呈浓度或时间依赖性抑制细胞增殖、减少细胞集落的形成,降低细胞的迁移能力,同时能显著提高HeLa细胞的凋亡率,影响细胞凋亡相关蛋白(Bcl-2、Bax、Erk、P38和Procaspase-3等)的表达.Blf-ADT对人宫颈癌HeLa细胞的生长有显著的抑制作用,对治疗宫颈癌具有潜在的临床应用价值.
- Abstract:
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The purpose of this study was to observe the activity and mechanisms of a new compound Blf-ADT on HeLa cells. The effects of Blf-ADT on Hela cells was investigated by morphological observation,MTT assay and colony formation assay,respectively. Wound healing assay was used to detect the effect of Blf-ADT on the migration of HeLa cells. Meanwhile,the activities of Blf-ADT on apoptosis was measured by Flow cytometry. Furthermore,Western blot was performed to investigate the expressions of related proteins in HeLa cell line treated with Blf-ADT. As a result,we found that Blf-ADT could inhibit cell proliferation and reduce cell migration in a dose-and time-dependent manner. In addition,Blf-ADT could significantly increase the rate of apoptosis and affect the expression of apoptosis-related proteins. Taken together,the data indicate that Blf-ADT can significantly inhibit the growth of HeLa cells and have potential clinical value for treatment of cervical cancer.
参考文献/References:
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备注/Memo
- 备注/Memo:
-
收稿日期:2015-06-03.
基金项目:教育部留学回国人员科研启动基金资助项目(教外司留[2015]311号)、南京师范大学留学回国人员启动基金项目.
通讯联系人:绪广林,副教授,研究方向:气体信号分子供体物的抗炎及抗肿瘤作用. E-mail:xudunlop@126.com
doi:10.3969/j.issn.1001-4616.2016.01.012
更新日期/Last Update:
2016-03-30