[1]王 倩*,潘 望,李 伟*,等.Blf-ADT对HeLa细胞的抑制作用及其机制的研究[J].南京师范大学学报(自然科学版),2016,39(01):67.
 Wang Qian,Pan Wang,Li Wei,et al.Study on Anti-Tumor Activity and Mechanisms of Blf-ADT[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(01):67.
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Blf-ADT对HeLa细胞的抑制作用及其机制的研究()
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《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第39卷
期数:
2016年01期
页码:
67
栏目:
生命科学
出版日期:
2016-03-31

文章信息/Info

Title:
Study on Anti-Tumor Activity and Mechanisms of Blf-ADT
作者:
王 倩*潘 望李 伟*肖志一潘黎黎绪广林
南京师范大学生命科学学院,江苏省分子医学与生物技术重点实验室,江苏 南京 210023
Author(s):
Wang QianPan WangLi WeiXiao ZhiyiPan LiliXu Guanglin
School of Life Sciences,Nanjing Normal University,Jiangsu Province Key Laboratory for Molecularand Medical Biotechnology,Nanjing 210023,China
关键词:
HeLaBlf-ADT细胞增殖细胞凋亡抗肿瘤作用
Keywords:
HeLaBlf-ADTcell proliferationcell apoptosisanti-tumor effect
分类号:
Q4
文献标志码:
A
摘要:
研究Blf-ADT(布洛芬硫化氢供体物)的抗肿瘤作用,并对其机制作初步探讨.通过形态学观察、噻唑蓝(MTT)比色实验、集落形成实验测定HeLa细胞的增殖能力;细胞划痕实验测定HeLa细胞的迁移能力;Annexin V-FITC/PI双染流式细胞仪测定HeLa细胞的凋亡率;Western blotting检测细胞凋亡相关蛋白(Bcl-2、Bax、Erk、P38和Procaspase-3等)的表达.结果显示,Blf-ADT能呈浓度或时间依赖性抑制细胞增殖、减少细胞集落的形成,降低细胞的迁移能力,同时能显著提高HeLa细胞的凋亡率,影响细胞凋亡相关蛋白(Bcl-2、Bax、Erk、P38和Procaspase-3等)的表达.Blf-ADT对人宫颈癌HeLa细胞的生长有显著的抑制作用,对治疗宫颈癌具有潜在的临床应用价值.
Abstract:
The purpose of this study was to observe the activity and mechanisms of a new compound Blf-ADT on HeLa cells. The effects of Blf-ADT on Hela cells was investigated by morphological observation,MTT assay and colony formation assay,respectively. Wound healing assay was used to detect the effect of Blf-ADT on the migration of HeLa cells. Meanwhile,the activities of Blf-ADT on apoptosis was measured by Flow cytometry. Furthermore,Western blot was performed to investigate the expressions of related proteins in HeLa cell line treated with Blf-ADT. As a result,we found that Blf-ADT could inhibit cell proliferation and reduce cell migration in a dose-and time-dependent manner. In addition,Blf-ADT could significantly increase the rate of apoptosis and affect the expression of apoptosis-related proteins. Taken together,the data indicate that Blf-ADT can significantly inhibit the growth of HeLa cells and have potential clinical value for treatment of cervical cancer.

参考文献/References:

[1] LI X Y,WANG X. The role of human cervical cancer oncogene in cancer progression[J]. Int J Clin Exp Med,2015,15:8 363-8 368.
[2] 卢秀娥. 宫颈癌及癌前病变筛查现状及其研究进展[J]. 中国社区医师,2013,15:9.
[3] SAMBROOK J. Molecular cloning:a laboratory manual[M]. Beijing:Science Press,2008.
[4] SCHIFFMAN M H,BRINTON L A. The epidemiology of cervical carcinogenesis[J]. Cancer,2006,76:1 888-1 901.
[5] PANKIN D M,PISAN I P,FERLAY J. Estinates of the worldwide incidence of 25 major cancers[J]. Int Cancer,2007,80:827-841.
[6] ABBOTT R G,FORREST S,PIENTA K J. Simulating the hallmarks of cancer[J]. Artif Life,2006,12:617-634.
[7] ADAMS J M,CORY S. The Bcl-2 protein family:arbiters of cell survival[J]. Science,1998,281(5 381):1 322-1 326.
[8] 徐美华,张桂英,谢兆霞,等. 吲哚美辛对结肠癌细胞CDK2、CDK4、P21WAF/CIP1、Bcl-2及Bax蛋白表达的影响[J]. 中化杂志,2002,22:605-607.
[9] DEVERAUX Q L,REED J C. IAP family proteins-suppressors of apoptosis[J]. Genes Dev,1999,13:239-252.
[10] UEHLING D E,HARRIS P A. Recent progress on MAP kinase pathway inhibitors[J]. Bioorg Med Chem Lett,2015,25:4?047-4056.

备注/Memo

备注/Memo:
收稿日期:2015-06-03. 
基金项目:教育部留学回国人员科研启动基金资助项目(教外司留[2015]311号)、南京师范大学留学回国人员启动基金项目. 
通讯联系人:绪广林,副教授,研究方向:气体信号分子供体物的抗炎及抗肿瘤作用. E-mail:xudunlop@126.com
doi:10.3969/j.issn.1001-4616.2016.01.012
更新日期/Last Update: 2016-03-30