[1]齐雅玲,卢悟广,种丹阳,等.蛋白质异戊二烯化关键酶GGPPS结合小分子筛选模型的构建[J].南京师范大学学报(自然科学版),2017,40(04):87.[doi:10.3969/j.issn.1001-4616.2017.04.014]
　Qi Yaling,Lu Wuguang,Chong Danyang,et al.The Construction of Mevalonate Pathway Enzyme GGPPSProtein Binding Molecules Screening Model[J].Journal of Nanjing Normal University(Natural Science Edition),2017,40(04):87.[doi:10.3969/j.issn.1001-4616.2017.04.014]
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蛋白质异戊二烯化关键酶GGPPS结合小分子筛选模型的构建()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第40卷
期数:
2017年04期
页码:
87
栏目:
·生命科学·
出版日期:
2017-12-30

文章信息/Info

Title:
The Construction of Mevalonate Pathway Enzyme GGPPSProtein Binding Molecules Screening Model
文章编号:
1001-4616(2017)04-0087-06
作者:
齐雅玲1; 卢悟广2; 种丹阳1; 刘 佳1; 孙 倩1; 徐晓军3; 曹 鹏2; 方 雷1; 李朝军1; 薛 斌1
(1.南京大学医学院,医药生物技术国家重点实验室,江苏省医学分子技术重点实验室,江苏 南京 210093)(2.江苏省中西医结合医院,江苏 南京 210028)(3.中国药科大学,天然药物活性组分与药效国家重点实验室,江苏 南京 210009)
Author(s):
Qi Yaling1; Lu Wuguang2; Chong Danyang1; Liu Jia1; Sun Qian1; Xu Xiaojun3; Cao Peng2; Fang Lei1; Li Chaojun1; Xue Bin1
(1.State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Key Laboratory of Molecular Medicine,School of Medicine,Nanjing University,Nanjing 210093,China)(2.Jiangsu Province Hospital on Integration of Chinese and Western Medicine,Nanjing 21002
关键词:
GGPPS重组蛋白; 微量热泳动; 小分子筛选
Keywords:
GGPPS recombination protein; microscale thermophoresis; small molecular screening
分类号:
Q291
DOI:
10.3969/j.issn.1001-4616.2017.04.014
文献标志码:
A
摘要:
蛋白质的基本翻译后修饰包括磷酸化、糖基化、乙酰化、棕榈酸化等[1],而异戊二烯化修饰是蛋白质的基本翻译后修饰之一,属于蛋白质的脂质修饰[2]. 甲羟戊酸途径中的关键分支酶——香叶基香叶基焦磷酸合成酶(Geranylgeranyl diphosphate synthase,GGPPS)能够催化法尼基焦磷酸盐(Farnesyl diphosphate,FPP)生成香叶基香叶基焦磷酸盐(Geranylgeranyl diphosphate,GGPP),介导蛋白质异戊二
Abstract:
The post-translational modifications of proteins include phosphorylation,glycosylation,acetylation,palmitic acid and so on. And the prenylation modification of proteins is one of the basic post-translational lipid modifications of proteins. The key enzyme of mevalonate pathway,GGPPS,can catalyze FPP to generate GGPP and mediate the equilibrium of protein prenylation,affect disease progression including insulin resistance,hyperyension,abnormal reproductive metabolism. Hence,it has an important practical significance that construct a GGPPS interaction model to obtain its molecule therapeutic drugs. Obtain the high expression of EGFP fluorescent labeled GGPPS prokaryotic purified protein. Construct a GGPPS interaction model with microscale thermophoresis technology. The recombinant expression plasmid GGPPS-EGFP-His was constructed correctly verified by gel electrophoresis and sequencing verification.The purified recombinant protein reached a purity of about 80%. In the microscale thermophoresis assay,the GGPPS substrate farnesyl pyrophosphate concentration gradient test showed a good combination.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-06-24.
基金项目:国家自然科学基金(31371373)、江苏省自然科学基金(BK20151395)、天然药物活性组分与药效国家重点实验室开放基金(SKLNMKF201606)、中央高校基本科研业务费专项资金(021414380330).
通讯联系人:薛斌,博士,副教授,研究方向:代谢类疾病的调控机制、肝损伤再生恢复研究. E-mail:xuebin@nju.edu.cn
更新日期/Last Update: 2017-12-30