[1]宋蕾蕾,孙文晓,焦可欣,等.基于高通量培养分析稻虾共作模式土壤的细菌群落[J].南京师大学报(自然科学版),2024,(01):57-67.[doi:10.3969/j.issn.1001-4616.2024.01.008]
　Song Leilei,Sun Wenxiao,Jiao Kexin,et al.Comparison of Soil Bacterial Communities in Rice-Crayfish Cocropping Based on High-Throughput Culturable Method[J].Journal of Nanjing Normal University(Natural Science Edition),2024,(01):57-67.[doi:10.3969/j.issn.1001-4616.2024.01.008]
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基于高通量培养分析稻虾共作模式土壤的细菌群落()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
期数:
2024年01期
页码:
57-67
栏目:
生态学
出版日期:
2024-03-15

文章信息/Info

Title:
Comparison of Soil Bacterial Communities in Rice-Crayfish Cocropping Based on High-Throughput Culturable Method
文章编号:
1001-4616(2024)01-0057-11
作者:
宋蕾蕾1; 孙文晓1; 焦可欣1; 陈钰璐1; 邹淑琪1; 张淅月1; 徐晨伟2; 3; 张 杨1; 戴传超1
(1.南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省微生物与功能基因组学重点实验室,江苏 南京 210023)
(2.江苏省农业农村污染防治技术与装备工程研究中心,江苏 南通 226007)
(3.南通科技职业学院,江苏 南通 226007)
Author(s):
Song Leilei1; Sun Wenxiao1; Jiao Kexin1; Chen Yulu1; Zou Shuqi1; Zhang Xiyue1; Xu Chenwei2; 3; Zhang Yang1; Dai Chuanchao1
(1.School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Microbes and Functional Genomics,Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources,Nanjing 210023,China)
(2.Jiangsu Province Engineering Research Center of Agricultural and Rural Pollution Prevention Technology and Equipment,Nantong 226007,China)
(3.Nantong College of Science and Technology,Nantong 226007,China)
关键词:
高通量测序; 稻虾共作模式; 涂布培养法; 土壤微生物群落
Keywords:
high-throughput sequencing; rice-crayfish cocropping; spread plate method; soil bacterial communities
分类号:
S154.37
DOI:
10.3969/j.issn.1001-4616.2024.01.008
文献标志码:
A
摘要:
高通量测序技术的发展发现并鉴定了土壤微生物的未培养物种,然而,仅依赖非培养的高通量测序无法获得发挥关键功能的微生物培养物,限制了对其功能的验证及其应用,这对关键功能微生物的机理性研究和应用具有一定的局限性. 本研究以稻虾共作和水稻单作两种稻作模式的土壤样品为研究对象,分别对比了传统平板涂布、96孔培养板划线分离以及将非培养和培养法相结合的——“96孔高通量培养法”3种方法的土壤细菌分类差异、菌群多样性、组成特异性以及细菌功能特征. 结果表明,相对于传统涂布培养法,96孔高通量培养法所获取的细菌数量高约5倍,能鉴定到属的培养物高约4倍,预测功能鉴定提高约2倍. 由此可见,“96孔高通量培养法”大大提高了对土壤细菌培养物的获取和鉴定率. 本研究弥补了传统培养法的获得率低的不足,拓宽了高通量培养技术的应用领域,同时,也为进一步揭示稻虾共作模式参与土壤生态变化过程的微生物机制提供研究基础.
Abstract:
With the development of high-throughput sequencing technology,more and more unculturable species of soil microorganisms have been discovered and identified. However,high-throughput sequencing cannot isolate and purify a single strain with key functions from massive databases,which has certain limitations on the mechanical research and application of key function microorganisms. In this study,soil samples of rice-crayfish cocropping and rice-single cropping were taken as the research object. The differences were compared in soil bacterial classification,microbial diversity,composition specificity,and microbial functional characteristics of traditional spread plate method,96-well culture plate in situ scribing separation,and the combination of high-throughput and cultivable methods—the “96-well high-throughput cultivable method”. The results showed that comparing with the traditional spread plate method,the number of bacteria obtained by 96-well high-throughput culture method was increased by about 5 times,the identification of genus level was increased by about 4 times,and the identification of function was increased by about 2 times. It can be seen that the 96-well high-throughput culturable method greatly improves the “resolution” of the separation and identification of soil microorganisms. The results of this study made up for the shortage of strains that could not be obtained by traditional spread plate method,expanded the application field of high-throughput culturable technology,and also provided a research basis for further revealing the microbial mechanism involved in the soil ecological change process of rice-crayfish cocropping model.

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备注/Memo

备注/Memo:
收稿日期:2023-08-21.
基金项目:国家自然科学基金青年项目(32001210)、江苏省高校自然科学基金面上项目(22KJB180003)、江苏省农业农村污染防治技术与装备工程研究中心开放课题(GCZXZD2301)、江苏省级大学生创新创业训练项目(202210319101Y).
通讯作者:张杨,博士,讲师,研究方向:土壤微生物生态.E-mail:yangzhang@nnu.edu.cn
更新日期/Last Update: 2024-03-15