[1]程 滨,张娜娜,许 勤,等.溴氰菊酯抗感品系小菜蛾成虫差异表达蛋白的鉴定[J].南京师大学报(自然科学版),2010,33(02):87-90.
　Cheng Bin,Zhang Nana,Xu Qin,et al.Identification of Differentially Expressed Proteins Between Deltamethrin Resistant and Susceptible Strains of Adult Plutella xylostella(L.)[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(02):87-90.
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溴氰菊酯抗感品系小菜蛾成虫差异表达蛋白的鉴定()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年02期
页码:
87-90
栏目:
生命科学
出版日期:
2010-06-20

文章信息/Info

Title:
Identification of Differentially Expressed Proteins Between Deltamethrin Resistant and Susceptible Strains of Adult Plutella xylostella(L.)
作者:
程 滨1;  张娜娜2;  许 勤3;  杨小怡2;  程罗根2;  李忠英4
1. 淮南师范学院生命科学系, 安徽淮南232001 2. 南京师范大学生命科学学院, 江苏南京210046 3. 南京师范大学电气与自动化工程学院, 江苏南京210042 4. 贵州省农业科学院植物保护研究所, 贵州贵阳550006
Author(s):
Cheng Bin1; Zhang Nana2; Xu Qin3; Yang Xiaoyi2; Cheng Luogen2; Li Zhongying4
1.School of Life Science,Huainan Normal University,Huainan 232001,China 2. School of Life Science, Nan jing Norm alU n iversity, Nan jing 210046, China 3. School of E lectrical and Autom ation Engin eering, Nan jing Norma lU n ivers ity, Nan jing 210042,
关键词:
小菜蛾;  抗药性;  双向电泳;  质谱分析;  溴氰菊酯
Keywords:
P lutella xy lostella ( L. );  res istance;  two-d im ensiona l ge l electrophoresis;  MALDI-TOF-M S;  de ltam ethrin
分类号:
S433
摘要:
通过对小菜蛾Plutella xylostella(L.)抗溴氰菊酯品系和敏感品系成虫双向电泳图谱的比较,找到8个差异明显的点,并结合质谱分析技术和数据库信息检索最终鉴定出7个蛋白.其中包括2个代谢相关蛋白,2个调控蛋白,1个结构分子蛋白,其余2个蛋白质功能皆不明确.这些在抗性或敏感品系中差异表达蛋白质的发现,为进一步研究小菜蛾抗药性形成机制和抗性相关蛋白的寻找奠定了基础.
Abstract:
Tw o-dim ensiona l po lyacry lam ide ge l electrophoresis was used to ana lyze prote in changes in response to de ltam ethrin resistance in adult P lu tella xylo stella( L. ). A fter the analysis o f the spec trum o f gel e lectropho resis, e ight prote ins we re found different in expression betw een susceptible and de ltam e thr in-resistance stra ins. Comb ined w ith the app lica tion ofMALDI-TOF-M S and sim ilarity ana ly sis to the database, seven pro te insw ere identified successfu lly inc luding two m etabo lism-related prote ins, tw o regu la tory prote ins, one struc tura l prote in and tw o unknown prote in. These d ifferentia lly expressed prote ins m ay be related to the insecticide resistance in P lutella xy lostella ( L. ), so the resu lts lay a foundation fo r the further study o fm o lecu lar mechan ism s o f the resistance.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 安徽省教育厅重点项目(K J2008A137)、南京师范大学大学生实践创新计划项目.
通讯联系人: 程罗根, 博士, 教授, 硕士生导师. 研究方向: 遗传性领域. E-mail:chengluogen@njnu. edu. cn
更新日期/Last Update: 2013-04-08