[1]朱宇鹏,高九彩,赵碧玉,等.基于β-内酰胺酶的正向选择蛋白表达载体的构建[J].南京师大学报(自然科学版),2011,34(03):103-106.
 Zhu Yupeng,Gao Jiucai,Zhao Biyu,et al.Construction of Positive-Selection Protein Expression Vectors Based on β-Lactamase[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(03):103-106.
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基于β-内酰胺酶的正向选择蛋白表达载体的构建()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第34卷
期数:
2011年03期
页码:
103-106
栏目:
生命科学
出版日期:
2011-09-20

文章信息/Info

Title:
Construction of Positive-Selection Protein Expression Vectors Based on β-Lactamase
作者:
朱宇鹏高九彩赵碧玉尚广东
南京师范大学生命科学学院江苏省功能微生物与功能基因组学重点实验室,江苏南京210046
Author(s):
Zhu YupengGao JiucaiZhao BiyuShang Guangdong
Jiangsu Key Laboratory for Microbes and Functional Genomics,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
关键词:
正选择克隆载体蛋白表达麦芽糖结合蛋白β-内酰胺酶
Keywords:
positive selectioncloning vectorprotein expressionmaltose-binding proteinβ-lactamase
分类号:
Q78
摘要:
外源基因的高效克隆表达是分子生物学和生物化学研究的关键技术,在同时克隆多个基因至表达载体时的高通量操作时尤其重要,常规的基因克隆操作常常存在着很高的载体背景干扰.本研究报道了一种改进型β-内酰胺酶正向选择表达载体pPAE以及与麦芽糖结合蛋白融合的正向选择表达载体pPAE-MBP.实验证明pPAE和pPAE-MBP的克隆效率均高达100%,且目的蛋白均得到了有效的表达.pPAE和pPAE-MBP有作为常规尤其是高通量蛋白表达载体来使用的潜力.
Abstract:
High efficient gene cloning is the key factor for molecular biology and biochemical research; it is especially important for the manipulations of cloning numerous genes simultaneously,such as the high throughput cloning. Classic cloning method usually involves high background. This paper reports the construction and applications of improved positive selection protein expression vectors of pPAE and pPAE-MBP,with latter the target protein is fused with maltosebinding protein upon protein expression. Experiments showed that both pPAE and pPAE-MBP exhibited 100% cloning efficiency and the target proteins expressed well. pPAE and pPAE-MBP can be used as protein expression vectors in general work especially in the high throughput protein expression research.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:“重大新药创制”科技重大专项课题( 2009ZX091032674) .通讯联系人:尚广东,副教授,研究方向: 微生物药物的研发. E-mail: shanggd@ hotmail. Com
更新日期/Last Update: 2011-09-15