[1]林 英,肖 麒,林友福,等.小管福寿螺SNP标记开发及其在物种鉴定中的应用[J].南京师大学报(自然科学版),2021,44(03):96-102.[doi:10.3969/j.issn.1001-4616.2021.03.015]
　Lin Ying,Xiao Qi,Lin Youfu,et al.Development of SNP Markers for Pomacea canaliculata andApplication of Species Identification in P.maculata[J].Journal of Nanjing Normal University(Natural Science Edition),2021,44(03):96-102.[doi:10.3969/j.issn.1001-4616.2021.03.015]
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小管福寿螺SNP标记开发及其在物种鉴定中的应用()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第44卷
期数:
2021年03期
页码:
96-102
栏目:
·生物学·
出版日期:
2021-09-15

文章信息/Info

Title:
Development of SNP Markers for Pomacea canaliculata andApplication of Species Identification in P.maculata
文章编号:
1001-4616(2021)03-0096-07
作者:
林 英1; 肖 麒1; 林友福2; 李雪霞2; 殷文莉2; 李 宏1; 陈 炼2
(1.南京师范大学生命科学学院,江苏 南京 210023)(2.南京林业大学生物与环境学院,江苏 南京 210037)
Author(s):
Lin Ying1; Xiao Qi1; Lin Youfu2; Li Xuexia2; Yin Wenli2; Li Hong1; Chen Lian2
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China)
关键词:
小管福寿螺; 斑点福寿螺; 全基因组重测序; 单核苷酸多态性位点; 分子鉴定
Keywords:
Pomacea canaliculata; P.maculata; whole genome resequencing; SNPs; molecular identification
分类号:
K826.15
DOI:
10.3969/j.issn.1001-4616.2021.03.015
文献标志码:
A
摘要:
小管福寿螺(Pomacea canaliculata)是全球100种恶性外来入侵物种之一,对入侵地的经济和环境造成了严重危害. 研究发现,入侵中国的福寿螺除小管福寿螺外,还包括斑点福寿螺(P.maculata). 本研究基于全基因组重测序数据开发单核苷酸多态性标记(single nucleotide polymorphism,SNP)用于福寿螺的遗传多样性分析及物种鉴定. 利用Sanger测序法对开发的22个SNP位点在浙江丽水小管福寿螺群体中进行遗传多样性分析,结果表明观测杂合度和期望杂合度范围分别为0～0.933和0.033～0.549,多态信息含量(PIC)范围为0.032～0.466. 通过计算上述位点在小管福寿螺和斑点福寿螺之间分化指数(Fst)和等位基因频率,获得在两物种中存在明显差异的5个SNP位点用于物种鉴定. 在30个小管福寿螺和18个斑点福寿螺样本中进行分型验证,结果表明,5个SNP位点单独使用时物种鉴定准确率均较高(>84.21%),当联合使用3～5个位点时准确率可达到100%. 这些SNP位点将有助于福寿螺种群遗传学方面的研究以及小管福寿螺和斑点福寿螺高效、准确的物种鉴别.
Abstract:
Pomacea canaliculata is one of the 100 world’s worst invasive alien species,which causes serious economic and environmental damage in the invaded areas. Both P.canaliculata and P.maculata invaded most southern provinces in China. In order to investigate the population genetic structure and diversity of these taxa,22 single nucleotide polymorphism markers(SNP)were isolated from the whole genome sequencing data of Pomacea spp.. Polymorphisms of these SNPs were uated in P.canaliculata from Lishui City,Zhejiang Province. The results showed that the observed,expected heterozygosity and polymorphism information content per locus ranged from 0 to 0.933,0.033 to 0.549,0.032 to 0.466,respectively. Five SNPs were identified with the high Fst values and different allele frequency between P.canaliculata and P.maculata. Then these five diagnostic SNPs were genotyping in 30 samples of P.canaliculata and 18 samples of P.maculata. Species identification accuracy of single SNP locus was high(>84.21%)and the accuracy could reach 100% when three to five diagnostic SNPs were used simultaneously. Those polymorphic SNP markers will be helpful for population genetics study on P.canaliculata and efficient and accurate species identification of P.canaliculata and P.maculata.

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备注/Memo

备注/Memo:
收稿日期:2021-03-20.
基金项目:国家自然科学基金项目(31770402)、江苏省自然科学基金项目(BK20171407).
通讯作者:陈炼,博士,副教授,研究方向:入侵生物学. E-mail:chenlian_2004@163.com
更新日期/Last Update: 2021-09-15