[1]邵世光,等.基于正交实验法的铁皮石斛原球茎分化和生根条件研究[J].南京师大学报(自然科学版),2009,32(04):98-102.
　Shao Shiguang,Hou Beiwei,Zhou Qi,et al.Studies on Condition Protocorm Differentiation and Root Induction of Dendrobium officinale by Orthogonal Design[J].Journal of Nanjing Normal University(Natural Science Edition),2009,32(04):98-102.
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基于正交实验法的铁皮石斛原球茎分化和生根条件研究()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第32卷
期数:
2009年04期
页码:
98-102
栏目:
生命科学
出版日期:
2009-12-30

文章信息/Info

Title:
Studies on Condition Protocorm Differentiation and Root Induction of Dendrobium officinale by Orthogonal Design
作者:
邵世光1;  2;  侯北伟1;  周琪1;  张潇潇1;  卢蓓蓓1;  柏伟娅1;  丁小余1
1. 南京师范大学生命科学学院, 江苏南京210046
 2. 连云港师范高等专科学校, 江苏连云港222006)
Author(s):
Shao Shiguang1; 2; Hou Beiwei1; Zhou Qi1; Zhang Xiaoxiao1; Lu Beibei1; Bai Weiya1; Ding Xiaoyu1
1.School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
关键词:
铁皮石斛;  组织培养;  原球茎分化;  生根;  正交实验
Keywords:
Dendrobium officinale;  tissue cu lture;  protocorm d ifferentiation;  roo t induction;  orthogonal expe rim en t
分类号:
S567.239
摘要:
利用6-BA、IBA、pH和培养基种类、琼脂浓度、KCl浓度各三个因素三水平进行L9(34)正交实验,研究各因素对铁皮石斛原球茎分化和生根的影响.对实验统计通过极差分析及方差分析,结果表明:在基本培养基N6中,当6-BA的浓度为2 mg/L、IBA浓度为0.2 mg/L、pH值为5.8时,原球茎最易于分化成丛生小苗;影响原球茎分化程度的因素依次为IBA浓度>6-BA浓度>pH值.铁皮石斛组培丛生苗生根的最优配方是:N6+2.5 mol/L KCl+4 g/L琼脂;影响诱导生根的主要因素是KCl浓度, 其次是琼脂浓度, 最后是基本培养基种类.
Abstract:
To study the effects of d ifferent fac to rs on pro tocorm differentiation and roo t induction o fDendrobium off icinale, tw o g roups of three kinds of facto rs on three levels we re used by o rthogona l des ign. The dataw ere analyzed w ith rang e ana ly sis and va riance analysis. Resu lts show ed that the optim alm edia to induce cluster shee t from protocorm w as N6 medium w ith 6-BA 2m g /L and IBA 0.2m g /L and the pH 5. 8. The effic iency o f the three facto rs w as in sequence of IBA > 6-BA > pH. On the o ther hand, N6 m edium w ith KC l 2.5 m ol/L and agar 4 g /L was su itab le for roo t induction o f Dendrobium officinale. Them ain factorwh ich influenced roo t induc tion was KC l concen tration, and the o the rs m ino r factors w ere ag ar concentra tion and type of basicm edium.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 30870234)、国家基础科学人才培养基金( J0730650)、江苏省自然科学基金( BK2008431 )资助项目.
通讯联系人: 丁小余, 博士, 教授, 博士生导师, 研究方向: 植物资源与生物技术. E-m a il:d ingxynj@263. net
更新日期/Last Update: 2013-04-23