[1]马长艳,等.用cDNA芯片技术筛选中华绒鳌蟹卵巢发育相关基因[J].南京师大学报(自然科学版),2010,33(03):76-80.
　Ma Changyan,Guo Yujie,Zhou Kaiya.Screening the Differentially Expressed Genes Related to the Ovarian Development of Chinese Mitten Crab(Eriocheir japonica sinensis) Using cDNA Macroarray[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(03):76-80.
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用cDNA芯片技术筛选中华绒鳌蟹卵巢发育相关基因()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年03期
页码:
76-80
栏目:
生命科学
出版日期:
2010-09-20

文章信息/Info

Title:
Screening the Differentially Expressed Genes Related to the Ovarian Development of Chinese Mitten Crab(Eriocheir japonica sinensis) Using cDNA Macroarray
作者:
马长艳1;  2;  郭豫杰1;  周开亚1
1. 南京师范大学生命科学学院, 江苏省生物多样性和生物技术重点实验室, 江苏南京210046 2. 南京医科大学发育遗传学系, 江苏南京210029
Author(s):
Ma Changyan 1; 2; Guo Yujie 1; Zhou Kaiya 1
1.Jiangsu Key Laboratory for Biodiversity and Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China 2. Department of Developm ental Genetics, Nan jing M ed icalU n ivers ity, N an jing 210029, Ch ina
关键词:
中华绒螯蟹;  卵巢;  抑制性差减杂交;  cDNA 芯片;  表达序列标签
Keywords:
E riocheir jap onica s inensis;  ovary;  suppression subtractiv e hybr id ization ( SSH );  cDNA macroarray;  expressed sequence tag
分类号:
S917.4
摘要:
为了克隆中华绒螯蟹卵巢发育相关基因, 我们构建了中华绒螯蟹卵巢差减cDNA文库. 本研究用cDNA 芯片技术对中华绒螯蟹卵巢差减cDNA 文库进行了初步筛选, 并从cDNA 芯片筛选的正向差减文库中随机选取 50个克隆进行序列测定. 结果共获得2倍以上差异表达克隆167个, 其中Ⅲ期高表达克隆104个,Ⅱ 期高表达克隆63个. 正向差减文库中共获得7个独立的EST( expressed sequence tag, EST ). 同源性分析结果表明, 这些EST 皆为新的EST, dbEST 登录号分别为: CA591892、CA591893、CA591894、CA591895、CA591896、CA591897 和 CA591898. 本研究结果为进一步克隆中华绒螯蟹卵巢发育相关基因的全长cDNA 序列并研究其功能, 阐明中华绒螯蟹卵巢发育的分子机理奠定了重要的前期工作基础.
Abstract:
To c lone genes w hich a re re lated to the ovar ian deve lopm ent of Chinesem itten crab, the sub trac ted libra ries have prev iously been construc ted. In the present study, the cDNA m acroarray was prepared to screen the diffe rentia lly expressed g enes and fifty clones identified from the fo rw ard subtrac ted cDNA libra ry w ere selected random ly and sequenced. In tota ,l 167 c lones whose signa l d iffe rence w as > 2 fo lds we re obta ined. O f the d ifferentially expressed clones, 104 c lones w ere h ighly expressed in the ovary at stageⅢ  , 63 c lones w ere h ighly expressed in the ovary a t stage Ⅱ . M oreover, seven independent ESTs we re obta ined from the fo rw ard sub trac ted cDNA library. H om o logy analysis showed that there aren t’ sequences significantlym atching to these ESTs. So theyw ere deduced to be nove l ESTs, and a ll o f them were deposited in dbEST. The accession num be rs are: CA591892、CA591893、CA591894、CA591895、 CA591896、CA591897 and CA591898, respec tive ly.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 30870286)、国家自然科学基金重点项目( 30630010) . 通讯联系人: 周开亚, 教授, 研究方向: 动物学. E-ma il:kyzhounj@126. com
更新日期/Last Update: 2013-04-08