[1]杜亚琼,张 宏,李凤良,等.溴氰菊酯胁迫下小菜蛾泛素化蛋白的分析与鉴定[J].南京师大学报(自然科学版),2013,36(02):108-112.
　Du Yaqiong,Zhang Hong,Li Fengliang,et al.Changes of the Ubiquitinated Proteins Stressed Under the Deltamethrin in Diamondback Moth,Plutella Xylostella L.[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(02):108-112.
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溴氰菊酯胁迫下小菜蛾泛素化蛋白的分析与鉴定()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年02期
页码:
108-112
栏目:
生命科学
出版日期:
2013-06-30

文章信息/Info

Title:
Changes of the Ubiquitinated Proteins Stressed Under the Deltamethrin in Diamondback Moth,Plutella Xylostella L.
文章编号:
1001-4616(2013)02-0108-05
作者:
杜亚琼1; 张 宏1; 李凤良2; 程罗根1
1.南京师范大学生命科学学院,江苏 南京 210023
2.贵州省农业科学院植保所,贵州 贵阳 550006
Author(s):
Du Yaqiong1; Zhang Hong1; Li Fengliang2; Cheng Luogen1
1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
2.Institute of Plant Protection,Guizhou Academy of Agriculture Science,Guiyang 550006,China
关键词:
小菜蛾; 溴氰菊酯; 泛素化蛋白; 差异
Keywords:
Plutella xylostella; Ubiquitin gene family; RNAi; gene silencing
分类号:
S481+4
文献标志码:
A
摘要:
利用聚丙烯酰胺凝胶电泳、Western Blot和LC MS/MS技术,对在溴氰菊酯胁迫下的小菜蛾(Plutella xylostella)四龄幼虫的泛素化蛋白(Ubiquitinated protein)的组分进行分析,结果表明小菜蛾溴氰菊酯敏感品系与小菜蛾溴氰菊酯抗性品系里的泛素化蛋白表达量有显著差异.本实验成功证明了在药剂胁迫下小菜蛾泛素化蛋白存在表达上的差异,该研究结果为分析昆虫细胞中泛素的功能及其对昆虫生理、生化和环境胁迫等方面的调节作用提供了参考.
Abstract:
The aim of this study was to analyse the Ubiquitinated protein in the 4rd larvae of Plutella xylostella under the stress of deltamethrin using SDS-PAGE,Western Blot and LC MS/MS.The results revealed significant differences in Ubiquitinated protein expression between deltamethrin-resistant strain and deltamethrin-sensitive strain of Plutella xylostella.It is successfully proved the differences in Ubiquitinated protein expression of Plutella xylostella under the stress of deltamethrin.The study provides a reference for analysing the function of Ubiquitin in the insect cells and the regulation function of it in physiology,biochemistry and environmental stress.

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相似文献/References:

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　Zhang Nana,Xu Qin,Li Fengliang,et al.Establishment of a Proteomic Analysis System by 2-DE From Adult Plutella Xylostella[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(02):78.
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[6]张晓飞,程罗根,陈之浩,等.小菜蛾对杀虫双抗性遗传的RDA分析[J].南京师大学报(自然科学版),2003,26(01):78.
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[7]张 宏,杜亚琼,李凤良,等.小菜蛾泛素基因的克隆与分析[J].南京师大学报(自然科学版),2013,36(03):116.
　Zhang Hong,Du Yaqiong,Li Fengliang,et al.Cloning and Analysis of Ubiqitin Genes of Plutella Xylostella(L.)[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(02):116.
[8]焦东旭,李 娜,程 晨,等.溴氰菊酯诱导果蝇细胞毒性及相关基因表达的变化[J].南京师大学报(自然科学版),2014,37(02):106.
　Jiao Dongxu,Li Na,Cheng Chen,et al.Induction of the Cytotoxicity and the Relative Genes Expression Response of Deltamethrin in Drosophila Kc cells[J].Journal of Nanjing Normal University(Natural Science Edition),2014,37(02):106.

备注/Memo

备注/Memo:
收稿日期:2013-01-23.
基金项目:国家自然科学基金(31071974).
通讯联系人:程罗根,博士,教授,研究方向:分子遗传学,遗传毒理学.E-mail:chengluogen@njnu.edu.cn
更新日期/Last Update: 2013-06-30