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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2008年01期

Page:

93-99

Research Field:

生命科学

Publishing date:

Info

Title:

Effects of Copper Stress on Mineral Nutrient,Protective Enzyme Activities and Their Isozyme Expression in Leaf of Hydrocharis dubia

Author(s):

Ji Wangdong;  Shi Guoxin;  Xu Qinsong;  Xu Ye;  Zhang Hui;  Zhao Juan

School of Life Science, Nanjing Normal University, Nanjing 210046, China

Keywords:

H ydro charis dubia;  Cu ²⁺ stress;  m ineral nutrient;  pro tective enzym e;  iso zym e

PACS:

Q945

DOI:

-

Abstract:

Hydrocharis dub ia plants exposed to 0, 2, 4, 6, 8m g /L Cu ²⁺ fo r 7 d w ere ana lyzed w ith re ference to its tox ic im pact on m inera l nutr ient adsorption, active oxygen generation, protec tive enzym e activ ities and the ir iso zym e express ion. The resu lts showed that, w ith the rise of Cu ²⁺ concentration in culturem edium: ( 1) them ineral nu trien t abso rption w as affected by Cu ²⁺ stress, as it increasd the abso rption of Ca ²⁺ 、Fe ³⁺ 、M g ²⁺ and Zn ²⁺ , and reduced tha t o f M n ²⁺ 、P and K+ , w ith increasing the concentration o f Cu ²⁺ . ( 2) The am ount and intensity o f prote in / peptide band dec reased gradua lly w ith augm ent of Cu2+ concentration w as obse rved in SDS- PAGE, respectively, and m any po lypeptides d isappeared sign ificantly in fronds treated w ith Cu2+ stress. ( 3) GR activ ity and H2O2 content change tendency assume the sing le peak curv e, in Cu ²⁺ concentration to 6 m g /L, the ir da tum up to m ax im ize, respec tive ly be tter 2. 51 tim es and 3.74 tim es than the con tro .l The O₂ - con tent andMDA content chang e assum e first slow, and then fast trend o f esca la tion, when Cu ²⁺ concentration to 8m g /L, the ir datum reach ing the c rest, compar ing 342.3% and 334. 2% to the contro;l SOD activ ity assumes the drop tendency, POD ac tiv ity assum es the trend of esca la tion, and CAT activ ity only has sligh tly rise. ( 4) Their isoenzym ic spectrum analysis demonstra tion, along w ith Cu ²⁺ concentra tion increasing, the SOD isoenzym ic band number and the expression quan tity reduces. The POD isoenzym ic band number gradua lly inc reases, increasing from 4 be lts to 7 be lts, and the expression quantity a lso presen ts the trend o f esca la tion. The CAT isoenzym ic band number does no t obv iously change, and the expression quantity has slight d iffe rence. The conc lusion could be reached that the death of plant resulted from destruction of structure founda tion o f physio log ica l function, unbaance o f ion equilib rium and d iso rder o f physio log ica lm etabo lism. The letha l concentra tion scope of Cu ²⁺ toHydrocharis dubia was 2~ 4m g /L based on the expe rim en t results

References:

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Last Update: 2013-05-05