|Table of Contents|

Genetic Diversity Study on Wild Populations of Apoacynum venetum Using RAPD Marker(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2008年04期
Page:
92-96
Research Field:
生命科学
Publishing date:

Info

Title:
Genetic Diversity Study on Wild Populations of Apoacynum venetum Using RAPD Marker
Author(s):
Peng Xuemei1Zhang Weiming12Wang Yangyang1Gu Gongping2Lu Changmei1
1.School of Life Science,Nanjing Normal University,Nanjing 210046,China
2. Nan jing Inst itu te for the Com prehens iveU t ilizat ion ofW ild Plant, Nan jing 210042, Ch ina
Keywords:
Apocynum venetum w ild population genetic d iversity RAPD
PACS:
S567.239
DOI:
-
Abstract:
Genetic diversity of Ap ocy num venetum was studied by the m ethod of random amp lified polym orph ic DNA ( RAPD) in this paper. Out o f 9 w ild popu lations from 8 prov inces o f Ch ina, 157 bands we re produced w ith 17 random pr im ers and 108 of them w ere po lym orphic. A t spec ies leve,l its po lymo rph ic lo ci percentage, N ei’s genetic d iversity index and Shannon’s in fo rm ation index w ere 68.79%, 0. 229 6 and 0. 339 0 respectively. The tota l gene d ifferen tiation ( G st) ofA. venetum w as 0.884 1. Th is ind icated that 881 41% o f genetic var iation res ided am ong popu lations and genetic va riation among populations w asm uch h ighe r than thatw ithin popu la tions. Results from genetic d istances and cluster ana lys is showed that relationsh ips am ong populations was not comp le tely co rre lated w ith their geog raph ica l d istances. According to the current genetic d iversity s ituation o fA. venetum, protec tion of natural population w as suggested to be streng thened.

References:

[ 1] 张绍武, 胡瑞林, 钱学射. 我国罗布麻分布区的地理区划[ J] . 中国野生植物资源, 2000, 19( 4): 20-22.
[ 2] 薛华茂, 钱学射, 张卫明, 等. 罗布麻的化学成分研究进展[ J]. 中国野生植物资源, 2005, 24( 4): 6-8, 12.
[ 3] 国家药典委员会. 中华人民共和国药典[ S]. 北京: 化学工业出版社, 2000.
[ 4] 左志锐, 穆鼎, 高俊平, 等. 百合遗传多样性及亲缘关系的RAPD分析[ J]. 园艺学报, 2005, 32( 3): 468-472.
[ 5] 李景欣, 乌仁图雅, 乌日娜. RAPD分子标记技术在植物遗传多样性研究中的应用[ J]. 畜牧与饲料科学, 2004, 6:57-58.
[ 6] Lim W, Mudge K W, W eston L A. U tiliza tion o f RAPD m arke rs to assess genetic d iversity o f w ild popu lations o f North American g inseng ( Panax qu inque folium ) [ J]. PlantaM edica, 2007, 73( 1): 71-76.
[ 7] 张卫明, 王曼丽, 彭雪梅, 等. 三种/ 罗布麻0干燥叶片基因组DNA 提取方法[ J] . 南京师大学报: 自然科学版,2007, 30( 1): 102-105.
[ 8] 邹喻苹, 葛颂, 王晓东, 等. 系统与进化植物学中的分子标记[M ] . 北京: 科学出版社, 2001: 140-149.
[ 9] 李太武, 李成华, 宋林生, 等. 5个泥蚶群体遗传多样性的RAPD分析[ J] . 生物多样性, 2003, 11( 2): 118-124.
[ 10] 李巧明, 赵建立. 云南干热河谷地区余甘子居群的遗传多样性研究[ J] . 生物多样性, 2007, 15( 1): 84-91.
[ 11] B rown A H D, Burdon J J, Jaro sz A M. Isozym e ana lys is o f plan tm ating system [ C ] / / Isozym es in Plant B io logy, Portland: Dioscor ides Press, 1989: 73-86.
[ 12] 潘庆杰, 李正红, 王雁, 等. 地涌金莲野生与栽培种群遗传多样性RAPD分析[ J] . 林业科学研究, 2007, 20( 5):668-672.
[ 13] 王瑷琦, 黄璐琦, 邵爱娟, 等. 孑遗植物杜仲的遗传多样性RAPD分析和保护策略研究[ J] . 中国中药杂志, 2006,31( 19): 1 583-1 586.
[ 14] 张吉宇, 袁庆华, 王彦荣, 等. 胡枝子属植物野生居群遗传多样性RAPD分析[ J]. 草地学报, 2006, 14( 3): 214-218.
[ 15] 周丽, 王效山, 黄和平, 等. 不同产地罗布麻叶总黄酮的含量测定[ J] . 现代中药研究与实践, 2005, 19( 5): 37-38

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Last Update: 2013-05-05