|Table of Contents|

Isolation Different PML Isoform by PCR Analysis(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2009年03期
Page:
88-91
Research Field:
生命科学
Publishing date:

Info

Title:
Isolation Different PML Isoform by PCR Analysis
Author(s):
Shen Yuqing; Xia Mei; Miao Fengqing; Xie Wei; Zhang Jianqiong
The Key Laboratory of Developmental Genes and Human Disease,Ministry of Education,Department of Genetics and Developmental Biology,Southeast University Medical School,Nanjing 210009,China
Keywords:
PML gene;  iso form;  PCR
PACS:
R733.7
DOI:
-
Abstract:
PM L ( Prom yelocy tic Leukem ia) gene is located on chrom osom e 15 and com po sed of n ine exons. Mo re than twenty d ifferen t PML iso form s have been discovered and categorized in to seven g roups by diffe rent splic ing pattern. In order to indentify different isoforms o f the ind iv idua l g roups, five pa irs of prim er were designed to be used for iso form-spec ific PCR am plification acco rd ing to the sequences at theC-term ina l o f PML gene. Analysis of the diffe rent iso form s w as based on the presence and the s ize o f the PCR product am plified by using a ll the five pairs of pr im ers. Contro l exper-i m ent w as done by analysis o f a pc DNA3.1-GFP-PML plasm id wh ich is known to be PMLIV. In add ition, PML iso form in a liver cancer ce ll line QGY7701 w as determ ined to be a m ixture of PMLII and PM LIV by the sam e way. In conc lus ion, PML isoform o f the seven g roups can be c lassified by sim ply PCR amp lification using the des igned five pa irs o f pr im ers.

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Last Update: 2013-04-23