|Table of Contents|

eIF4E Promotes CXCL12 Induced Metastasis of Gastric Cancer(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2015年02期
Page:
93-
Research Field:
生命科学
Publishing date:

Info

Title:
eIF4E Promotes CXCL12 Induced Metastasis of Gastric Cancer
Author(s):
Wu Dandan; Ni Ping; Sun Geng; He Wenping; Zhai Sulan; Li Ping; Zou Meijuan; Wang Xuerong
Department of Pharmacology,School of Medicine,Nanjing Medical University,Nanjing 210029,China
Keywords:
CXCL12; eIF4E; morphological changes; migration; gastric cancer; MMP9
PACS:
R739.91
DOI:
-
Abstract:
To study of eIF4E in CXCL12 induced morphological changes and migration mechanism in gastric cancer cells. In gastric cancer cell lines(SGC7901,MGC803),cell morphological changes with CXCL12 treatment were observed under microscopy. The effect of CXCL12 on gastric cancer cell migration was determined by transwell assay. Western blot anlaysis was used to detect the eIF4E phosphorylation and total protein levels in SGC7901 treated with CXCL12. MMP9 mRNA expression was determined by qRT-PCR assay. Results show that,CXCL12 induced gastric cancer cells from epithelial morphology to mesenchymal morphology as well as increased migration. Morphological changes and migratory capacity induced by CXCL12 were inhibited by eIF4E siRNA transfection in SGC7901 cells. In addition,CXCL12 induced upregulation of MMP9 mRNA level. Conclusion:eIF4E is involved in CXCL12 induced the morphological changes and cell migration of gastric cancer cells possibility through upregulation of MMP9.

References:

[1] International Agency For Research on Cancer. GLOBOCAN 2012:Estimated incidence,mortality and revalence worldwide in 2012[EB/OL]. [2014-10-25]http://globocan.iarc.fr/old/FactSheets/cancers/stomach-new.asp.
[2]Milne A N,Carneiro F,O’Morain C,et al. Nature meets nurture:molecular genetics of gastric cancer[J]. Hum Genet,2009,126(5):615-628.
[3]Orditura M,De Vita F,Muto P,et al. Adjuvant chemoradiotherapy in patients with stage Ⅲ or Ⅳ radically resected gastric cancer:a pilot study[J]. Arch Surg,2010,145(3):233-238.
[4]Lee H J,Kim S W,Kim H Y,et al. Chemokine receptor CXCR4 expression,function,and clinical implications in gastric cancer[J]. Int J Oncol,2009,34(2):473-480.
[5]Sun X,Cheng G,Hao M,et al. CXCL12/CXCR4/CXCR7 chemokine axis and cancer progression[J]. Cancer Metastasis Rev,2010,29(4):709-722.
[6]Teicher B A,Fricker S P. CXCL12(SDF-1)/CXCR4 pathway in cancer[J]. Clin Cancer Res,2010,16(11):2 927-2 931.
[7]Ying J,Xu Q,Zhang G,et al. The expression of CXCL12 and CXCR4 in gastric cancer and their correlation to lymph node metastasis[J]. Med Oncol,2012,29(3):1 716-1 722.
[8]Yasumoto K,Koizumi K,Kawashima A,et al. Role of the CXCL12/CXCR4 axis in peritoneal carcinomatosis of gastric cancer[J]. Cancer Res,2006,66(4):2 181-2 187.
[9]De Benedetti A,Graff J R. eIF4E expression and its role in malignancies and metastases[J]. Oncogene,2004,23(18):3 189-3 199.
[10]Martineau Y,Azar R,Bousquet C,et al. Anti-oncogenic potential of the eIF4E-binding proteins[J]. Oncogene,2013,32(6):671-677.
[11]Graff J R,Konicek B W,Carter J H,et al. Targeting the eukaryotic translation initiation factor 4E for cancer therapy[J]. Cancer Res,2008,68(3):631-634.
[12]Thumma S C,Kratzke R A. Translational control:a target for cancer therapy[J]. Cancer Lett,2007,258(1):1-8.
[13]Ma Z,Zhu L,Luo X,et al. Perifosine enhances mTORC1-targeted cancer therapy by activation of GSK3β in NSCLC cells[J]. Cancer Biol Ther,2012,13(11):1 009-1 017.
[14]Mathis J M,Williams B J,Sibley D A,et al. Cancer-specific targeting of an adenovirus-delivered herpes simplex virus thymidine kinase suicide gene using translational control[J]. J Gene Med,2006,8(9):1 105-1 120.
[15]Nowak D E,Tian B,Jamaluddin M,et al. RelA Ser276 phosphorylation is required for activation of a subset of NF-kappaB-dependent genes by recruiting cyclin-dependent kinase 9/cyclin T1 complexes[J]. Mol Cell Biol,2008,28(11):3 623-3 638.
[16]Chen G,Chen S M,Wang X,et al. Inhibition of chemokine(CXC motif)ligand 12/chemokine(CXC motif)receptor 4 axis(CXCL12/CXCR4)-mediated cell migration by targeting mammalian target of rapamycin(mTOR)pathway in human gastric carcinoma cells[J]. J Biol Chem,2012,287(15):12 132-12 141.
[17]Hashimoto I,Koizumi K,Tatematsu M,et al. Blocking on the CXCR4/mTOR signalling pathway induces the anti-metastatic properties and autophagic cell death in peritoneal disseminated gastric cancer cells[J]. Eur J Cancer,2008,44(7):1 022-1 029.
[18]Monterrubio M,Mellado M,Carrera A C,et al. PI3Kgamma activation by CXCL12 regulates tumor cell adhesion and invasion[J]. Biochem Biophys Res Commun,2009,388(2):199-204.
[19]Sampieri C L,de la P S,Ochoa-Lara M,et al. Expression of matrix metalloproteinases 2 and 9 in human gastric cancer and superficial gastritis[J]. World J Gastroenterol,2010,16(12):1 500-1 505.
[20]Balkwill F R. The chemokine system and cancer[J]. J Pathol,2012,226(2):148-157.

Memo

Memo:
-
Last Update: 2015-06-30