[1]裴凌鹏,崔箭.叶黄素对破骨细胞分化的影响[J].南京师大学报(自然科学版),2008,31(03):100-103.
　Pei Lingpeng,Cui Jian.Effect of Lutein on the Differentiation of Osteoclast Induced From Bone Marrow in Mice[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(03):100-103.
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叶黄素对破骨细胞分化的影响()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年03期
页码:
100-103
栏目:
生命科学
出版日期:
2008-09-30

文章信息/Info

Title:
Effect of Lutein on the Differentiation of Osteoclast Induced From Bone Marrow in Mice
作者:
裴凌鹏;崔箭;
中央民族大学少数民族传统医学中心, 北京100081
Author(s):
Pei Lingpeng; Cui Jian
Central University for Nationalities,Research Center of Minarity Traditional Medicine,Beijing 100081,China
关键词:
叶黄素;  破骨细胞;  细胞分化
Keywords:
lutein;  osteoc last;  differentiation
分类号:
Q25
摘要:
研究叶黄素对体外破骨细胞分化的影响.建立由骨保护素配体(RANKL)和巨噬细胞集落刺激因子(M-CSF)共同细胞因子的小鼠破骨细胞骨髓诱导体系,将不同浓度的叶黄素作用于破骨细胞.受试细胞分为对照组、叶黄素低剂量组(10-8 mol/L)、叶黄素中剂量组(10 -7 mol/L)和叶黄素高剂量组(10-6 mol/L),并设立空白对照组.7 d后取细胞玻片进行抗酒石酸酸性磷酸酶(TRAP)染色,观察破骨细胞并计数;取骨片进行甲苯胺蓝染色,光镜下统计骨吸收陷窝面积;测量抗酒石酸酸性磷酸酶(TRAP)活性以及破骨细胞表面NF- kB 活化受体( RANK) mRNA 表达量. 诱导培养的破骨细胞形态特征明显; 叶黄素中、高剂量组在细胞数量、TRAP活性上与对照组相比有明显统计学差异(P < 0. 05); 叶黄素各剂量组在( RANK ) mRNA表达量上与对照组相比有明显统计学差异( P < 0. 05), 且呈量效关系. 研究表明叶黄素可以抑制体外培养的破骨细胞分化.
Abstract:
Effect o f lute in on the d iffe rentia tion of osteo clast induced from bonem arrow were studied in v itro. M ononuclea r ce lls o f m ice bonem arrow w ere incubated w ith DMEM conta in ing macrophage co lony stimu la ting fac to r (M - CSF) and recepto r activato r of NF - kB lig and ( RANKL). The cu lture was treated w ith lute in of d ifferent concentrations ( 10- 8 m ol/L、10- 7 m o l/L、10- 6 mo l/L) , respective ly. The cu lture ce lls w ere fixed and w ere stained fo r tarta te-resistant ac id ic phospha tase ( TRAP) a fter 7d. The form ation of osteoc lasts w as quantified by counting the num be r of TRAP+ mu ltinuc lea r ce lls. The percentage of bone resorbed surface, TRAP activ ity and the expression o f receptor ac tivator o f NF- kB ( RANK) mRNA w ere ana ly sed. The appearance o f reduced m ice osteo clasts w as typica.l Com pared w ith the contro l g roup, the num be r of TRAP+ mu ltinuc lea r cells, TRAP activity and the percentage of bone surface in lute in m idd le-do se group and lu tein h igh-dose g roup sign ificantly decreased( P < 0.05) . But the expression o f RANK mRNA sign ifican tly increased( P < 0.05 ) by treated in different doses o f lute in. Lute in can inh ib it o steoc lasts d ifferentiation in v itro.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 30171169)资助项目.
通讯联系人: 裴凌鹏, 博士, 讲师, 研究方向: 天然药物化学与骨营养. E-m ail:lppei@hotma il.com
更新日期/Last Update: 2013-05-05