[1]马长艳,郭豫杰,周开亚,等.中华绒鳌蟹卵巢发育相关的新基因EJO3的克隆和特征分析(英文)[J].南京师大学报(自然科学版),2010,33(02):71-76.
　Ma Changyan,Guo Yujie,Zhou Kaiya.Molecular Cloning and Characterization of a Novel Gene,EJO3,Putatively Involved in the Ovarian Development of Chinese Mitten Crab(Eriocheir Japonica Sinensis)[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(02):71-76.
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中华绒鳌蟹卵巢发育相关的新基因EJO3的克隆和特征分析(英文)()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年02期
页码:
71-76
栏目:
生命科学
出版日期:
2010-06-20

文章信息/Info

Title:
Molecular Cloning and Characterization of a Novel Gene,EJO3,Putatively Involved in the Ovarian Development of Chinese Mitten Crab(Eriocheir Japonica Sinensis)
作者:
马长艳;郭豫杰;周开亚;
南京师范大学生命科学学院, 江苏省生物多样性和生物技术重点实验室, 江苏南京210097
Author(s):
Ma Changyan; Guo Yujie; Zhou Kaiya
Jiangsu Key Lab for Biodiversity and Biotechnology,School of Life Science,Nanjing Normal University,Nanjing 210097,China
关键词:
抑制性差减杂交;  cDNA芯片;  中华绒鳌蟹;  卵巢;  EJO3
Keywords:
suppression subtractive hybr idiza tion ( SSH );  cDNA m acroa rray;  Er io cheir japonica sinensis;  ovary;  EJO 3
分类号:
S966.16
摘要:
运用了抑制性差减杂交和cDNA芯片技术鉴定中华绒鳌蟹卵巢发育相关基因,并用5′和3′RACE的方法克隆了一个与Ⅱ期相比在卵巢发育的Ⅲ期高表达的新基因EJO3(GenBank登录号:AY185919).EJO3基因的开放阅读框(ORF)长1368 bp,编码455个氨基酸.从氨基酸序列推算的EJO3的等电点和相对分子质量分别为5.79和50 000.生物信息学分析表明,EJO3可能是一个含有VWD结构域的分泌蛋白.EJO3在Ⅱ期和Ⅲ期卵巢的差异表达用Northern blot进行了进一步验证.组织表达谱分析表明EJO3在卵巢高表达,在肠、心脏、肌肉和肝胰腺中弱表达或不表达.本研究结果为进一步深入研究中华绒鳌蟹卵巢发育的分子机制奠定了重要的前期工作基础.
Abstract:
In the present study, w e pe rfo rm ed suppression subtractive hybr id ization ( SSH ) comb ining cDNA m acro array to identify genes putative ly invo lved in the ovar ian dev elopment of Ch inese m itten crab. A nove l g ene, EJO3 ( GenBank accession number: AY185919) w hose expression is h ighe r at stage III than that at stage II w as c loned by 5‘and 3’ RACE. The ORF ( open reading fram e, ORF) o f the cDNA o fEJO 3 consisted of 1 368 bp encod ing 455 am ino acids. The pI/Mw deduced from the am ino ac ids sequence w as 5. 79 /50 000. Pro te in coded byEJO 3 genew as probab ly a sec retion pro te in w ith aVWD dom a in. The d ifferen tia l express ion o fEJO3 w as further confirm ed byNo rthern blot ana lys is. Express ion profile ana ly sis show ed that EJO3 is h ighly expressed in ova ry and the re is little or no express ion o fEJO3 in intestine, heart, m usc le and hepatopancreas. Results of this study have made an important foundation to further eluc-i date them o lecu lar m echan ism o f the ova rian deve lopm ent of Ch inese m itten crab.

参考文献/References:

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相似文献/References:

[1]胡晓旻,孙晓文,李喜文,等.抑制性差减杂交方法克隆重力适应相关基因[J].南京师大学报(自然科学版),2001,24(04):82.
　Hu Xiaomin,Sun Xiaowen,Li Xiwen,et al.Cloning Genes Associated with Adaptability to Changing Gravity Environment Using Suppression Subtractive Hybridization[J].Journal of Nanjing Normal University(Natural Science Edition),2001,24(02):82.

备注/Memo

备注/Memo:
Foundation item: National Natural Science Foundation of China(30870286) and Key Project(30630010) Corresponding author: Zhou Kaiya, professor, m ajored in zoo logy. E-mail:kyzhounj@ 126. com
更新日期/Last Update: 2013-04-08