[1]严洁,马晓燕,杜婕,等.中国壁虎属9物种的DNA条码技术[J].南京师大学报(自然科学版),2010,33(04):84-90.
　Yan Jie,Ma Xiaoyan,Du Jie,et al.DNA Barcoding of Nine Species of Gekko From China[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(04):84-90.
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中国壁虎属9物种的DNA条码技术()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年04期
页码:
84-90
栏目:
生命科学
出版日期:
2010-12-20

文章信息/Info

Title:
DNA Barcoding of Nine Species of Gekko From China
作者:
严洁;马晓燕;杜婕;周开亚;
江苏省生物多样性和生物技术重点实验室, 南京师范大学生命科学学院, 江苏南京210046
Author(s):
Yan Jie; Ma Xiaoyan; Du Jie; Zhou Kaiya
Jiangsu Key Laboratory for Biodiversity and Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
关键词:
DNA条码;  细胞色素c 氧化酶I;  壁虎属;  壁虎鉴定
Keywords:
DNA ba rcode;  cy tochrom e c ox idase subun it I;  Gekko;  gecko identification
分类号:
Q789
摘要:
测定了无蹼壁虎、粗疣壁虎和中国壁虎共17个样品的COI基因部分片段,结合其他已知序列,对我国9种壁虎的DNA条码序列进行比较分析,计算种间及种内平均遗传距离,并构建系统发生树.结果显示所有DNA条码都为各物种所独有,不存在物种之间的共享.属内种间差异明显大于种内差异,且同一物种的不同个体均聚成高支持率的单系群,提示该条码在壁虎属物种中有很好的识别能力,可以用于物种鉴定.DNA条码还为粗疣壁虎和铅山壁虎的物种有效性提供了有力的分子证据.
Abstract:
W e determ ined partial sequence of the cytochrom e c ox idase subun it I gene ( CO I) fo r 17 spec im ens from G ek- k o sw inhonis, G. scabridus and G. chinensis. Comb in ing that from ano ther 6 geckos, DNA ba rcodes for 9 spec ies ofG ek- k o from Ch inaw ere com pared. Average K imura-2-param eter differences be tw een and w ithin spec ies w ere calculated and the phy logenetic treesw ere reconstruc ted. Our results showed that a ll DNA barcodes we re speciesspec ific, that is no se quence w as shared in tw o spec ies. D ivergence betw een spec ies w as g rea ter than that ofw ithin species, and ind iv idua ls o f the sam e species w ere clustered as high ly supported m onophy le tic g roup, ind ica ting the po tential o f DNA barcoding for spec ies identification in genus Gekko. DNA barcodes a lso prov ide m o lecular ev idence suppo rting the valid ity o f Gekko scabr idus and G ekko hokouensis.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 31000949、30870286)、南京师范大学创新团队项目( 0319PM 0902) . 通讯联系人: 周开亚, 教授, 博士生导师, 研究方向: 动物学. Email:kyzhounj@126. com
更新日期/Last Update: 2013-04-08