[1]张娜娜,许 勤,李风良,等.小菜蛾成虫蛋白质组双向电泳图谱的建立及条件优化[J].南京师大学报(自然科学版),2011,34(02):78-82.
　Zhang Nana,Xu Qin,Li Fengliang,et al.Establishment of a Proteomic Analysis System by 2-DE From Adult Plutella Xylostella[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(02):78-82.
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小菜蛾成虫蛋白质组双向电泳图谱的建立及条件优化()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第34卷
期数:
2011年02期
页码:
78-82
栏目:
生命科学
出版日期:
2011-06-20

文章信息/Info

Title:
Establishment of a Proteomic Analysis System by 2-DE From Adult Plutella Xylostella
作者:
张娜娜1;  许 勤2;  李风良3;  程罗根1
( 1. 南京师范大学生命科学学院, 江苏南京210046) ( 2. 南京师范大学能源与机械工程学院, 江苏南京210042) ( 3. 贵州省农业科学院植保所, 贵州贵阳550006)
Author(s):
Zhang Nana1; Xu Qin2; Li Fengliang3; Cheng Luogen1
1.School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
关键词:
小菜蛾;  双向电泳;  蛋白质组学
Keywords:
Plutella xy lostella;  two dimensional electrophoresis;  proteom ics
分类号:
S433.4
摘要:
通过选择合适的pH范围及长度的胶条,并对蛋白样品上样量、等电聚焦伏小时数、银染时间及样品前处理等进行优化,建立了以双向电泳技术为基础的小菜蛾成虫蛋白质组学研究方法.优化结果表明使用17 cm、pH3~pH10的非线性胶条可以得到分辨率较高的胶图.该方法的建立为小菜蛾抗药性差异蛋白质组学研究奠定了基础.
Abstract:
Sam ple preparation, IEF, appropriate strips and s live r sta in ing w ere optim ized to get better 2- DE pro files o f adu ltP lutella xy lostella. The resu lts showed tha t 2- DE pro files w ith h igh reso lution w ere obta ined by using 17 cm pH 3 ~ pH 10 non linear strips. Th is study can lay a foundation for future study o f diffe rentia l proteom ics

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 31071974)、安徽省教育厅重点项目(K J2008A137) . 通讯联系人: 程罗根, 博士, 教授, 研究方向: 遗传性领域的教学. Email:chengluogen@ njnu. edu. cn
更新日期/Last Update: 2011-06-15